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Application of sodium lauryl sulphate ion pairing reversed phase HPLC in the separation of some isomeric and other closely related basic compounds in pharmaceutical analysis
Summary
Type:
HPLC
Application #:
104630
Catalog#:
720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection:
UV, 254nm
Conditions:
eluent: 0.01M Na lauryl sulphate in 0.0066M phosphate buffer pH 7.4 - acetonitrile (30:70)
flow rate 1ml/min
Authors:
Bidlo-Igloy M
Source:
J Chromatogr 520 (1990) 367 - 371
Details
Legend: Fig. 1. Chromatogram of a mixture of compounds a, b, and c (ratio 8:1:1), retention times a) 6.10, B) 7.42, C) 8.79min.
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